Contract Training: Hands-on cGMP Biomanufacturing of Vectors for Gene Therapy

NCTM 100 Discovery Drive, College Station, TX, United States

Receive hands-on training in the production of vectors for gene therapy products using an AAV2-GFP model system in a Sf9/Baculovirus system. Engage in upstream operations (suspension cell culture, vector production scale-up in single-use bioreactors); downstream operations (cell lysis, product recovery via depth filtration, purification using chromatography, and ultrafiltration for concentration and buffer exchange); and analytical […]

Open Enrollment: Advanced Downstream

NCTM 100 Discovery Drive, College Station, TX, United States

Learning Objectives• Protein Overview, Structure, Properties• Unicorn Software Overview, Method Editor, System Control• Ultrafiltration• Membrane Selection• TFF Process Development• NWP Measurements• GFP Concentration and Diafiltration• Flux vs. TMP curves for TFF Optimization• Cleaning Procedures for NWP Recovery• AKTA Avant Overview• Chromatography Basics• Column Packing• Chromatogram Evaluation• Step Design• Step vs. Gradient Elution• Dynamic Binding Capacity […]

$4578

Scaling up adherent cell culture with Corning® HYPERStack® vessels

NCTM 100 Discovery Drive, College Station, TX, United States

Learning Objectives During this course, learners will:Perform HYPERStack vessel manipulations including fill, empty, and dynamic incubate, which are used for cell seeding, media exchange, and harvest Observe a live-cell HYPERStack vessel harvest Understand HYPER technology and when to consider HYPERStack vs. traditional vessels Learn to work with closed-system tubing, which is pre-installed on the HYPERStack vessels Gain familiarity with other […]

$25