This professional development course examines the common applications of downstream processing in biopharmaceutical and vaccine manufacturing and principles behind them. Lecture and hands‐on training will cover the applications, platforms, equipment, and parameters that are critical to downstream bioprocessing. On Day 1, participants will learn about the different unit operations that are commonly used to manufacture biopharmaceuticals and vaccines, learn about cell lysis methods, and apply centrifugation principles. Day 2 will focus on different membrane filtration processes with hands-on sterile filtration and tangential flow filtration labs. The final day examines the principles of chromatography and scale‐up of chromatographic methods with the development and scale‐up of a separation method. Participants will receive 2.4 CEUs upon completion.