Receive hands-on training in the production of vectors for gene therapy products using an AAV2-GFP model system in a Sf9/Baculovirus system. Engage in upstream operations (suspension cell culture, vector production scale-up in single-use bioreactors); downstream operations (cell lysis, product recovery via depth filtration, purification using chromatography, and ultrafiltration for concentration and buffer exchange); and analytical […]

Learning Objectives • Overview of mRNA vaccines (background, applications, etc.)• Overview of cGMP and product quality• Invitro transcription of mRNA• Filtration• Chromatography• Lipid nanoparticle delivery systems• Cold chain• QC methods for mRNA vaccines• In vitro transcription reaction• UFDF• Lipid Nanoparticle Encapsulation• Analysis and quantification of mRNA  Register here

Learning Objectives • Overview of Cell Culture Principles • Cell Banking • Passaging of Adherent and Suspension Cells • Calculations for Transfection and Infection of Cell Cultures •Principles of Scale Up • Feeding Strategies and Recipe Design • Overview of PID Control Loops • Cell Culture Issues and Troubleshooting strategies • Growth Monitoring and Offline […]

Learning Objective  • Protein Overview, Structure, Properties • Unicorn Software Overview, Method Editor, System Control • Ultrafiltration • Membrane Selection • TFF Process Development • NWP Measurements • GFP Concentration and Diafiltration • Flux vs. TMP curves for TFF Optimization • Cleaning Procedures for NWP Recovery • AKTA Avant Overview • Chromatography Basics • Column […]

This week long, hands-on course provides a comprehensiveoverview of the techniques and challenges involved in the upstreamprocesses for manufacturing biologics.Lectures and laboratories cover biologic products, upstreamprocesses, biomass production, cell lines, bioreactors and theiroperation, and the analytics used to evaluate upstream production.Applied hands‐on training includes preparation and proper labelingof reagents and media, aseptic technique, plasmid extraction,purification […]