• Protein Overview, Structure, Properties
• Unicorn Software Overview, Method Editor, System Control
• Ultrafiltration
• Membrane Selection
• TFF Process Development
• NWP Measurements
• GFP Concentration and Diafiltration
• Flux vs. TMP curves for TFF Optimization
• Cleaning Procedures for NWP Recovery
• AKTA Avant Overview
• Chromatography Basics
• Column Packing
• Chromatogram Evaluation
• Step Design
• Step vs. Gradient Elution
• Dynamic Binding Capacity Determination
• Viral Clearance
• Scale-Up
• Packed Bed vs. Monolith Chromatography
• Anion Exchange Chromatography
• Hydrophobic Interaction Chromatography
