Participants will learn about working in a controlled environment, aseptic technique and how to operate basic laboratory equipment. Participants will then produce a model protein during a series of upstream activities, including bioreactor assembly, culture media preparation, batch cultivation, offline analysis and harvesting. Participants will also learn a series of downstream techniques to purify protein by performing buffer preparation, tangential flow filtration, column cleaning and sanitization, ion exchange and hydrophobic interaction chromatography.
