LEARNING OBJECTIVE Participants will learn about working in a controlled environment, aseptic technique and how to operate basic laboratory equipment. Participants will then produce a model protein during a series of upstream activities, including bioreactor assembly, culture media preparation, batch cultivation, offline analysis and harvesting. Participants will also learn a series of downstream techniques to […]

Receive hands-on training in the production of vectors for gene therapy products using an AAV2-GFP model system in a Sf9/Baculovirus system. Engage in upstream operations (suspension cell culture, vector production scale-up in single-use bioreactors); downstream operations (cell lysis, product recovery via depth filtration, purification using chromatography, and ultrafiltration for concentration and buffer exchange); and analytical […]